Figure 3: Expression of PGC-1α and AMPK mRNAs and protein in AngII-induced cardiac hypertrophy. (a) Expression of p-AMPK and AMPK protein in the control, AngII, and SSYX (0.25, 0.5, and 1.0 μg/ml) groups. (b) Expression of PGC-1α protein in each group. (c) Relative expression levels of AMPK mRNA in groups. (d) Relative expression levels of PGC-1α mRNA in groups. (e) Expression of p-AMPK and AMPK protein treated with 0.5 μg/ml SSYX for 0, 6, 12, 24, and 48 h. (f) Expression of PGC-1α protein in groups. (g) Relative expression levels of AMPK mRNA in groups; (h) relative expression levels of PGC-1α mRNA in groups. *P < 0.0001 versus control group; †P < 0.05 versus AngII group; *P < 0.0001 versus 0 h; the data are expressed as the mean ± SD (n = 5, samples were collected from five different passages). The symbols “*, †” indicate that the value of PGC-1α and AMPK mRNA expression significantly differed from those of different groups and different durations. p-AMPK: Activity AMP-activated protein kinase; AMPK: AMP-activated protein kinase; PGC-1α: Peroxisome proliferator-activated receptor coactivator 1 alpha; mRNA: Messenger RNA; Ang: Angiotensin; SD: Standard deviation; SSYX: Shensong Yangxin Capsule.