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  Indian J Med Microbiol
 

Figure 1: Myocardial hypertrophy and apoptosis in the control, AngII, SSYX (0.25, 0.5, and 1.0 μg/ml drug concentration) groups. (a) Representative immunofluorescent (α-actinin) images of NRCMs in each group. Bar = 200 μm. Red staining denotes α-actinin-stained cardiomyocytes. Blue staining denotes DAPI-stained myocardial cell nuclei. (b) Summarized data of surface area of myocardial cells in each group. (c) Relative expression levels of BNP mRNA in each group. (d) Relative expression levels of Capase-3 mRNA in each group. *P < 0.0001 versus the control group; P < 0.05 versus the AngII group. The data are expressed as the mean ± SD (n = 5, samples were collected from five different passages). The symbols “*, ” indicate that the value of cell volume and BNP and Capase-3 mRNA expression significantly differed from different groups. BNP: Brain natriuretic peptide; Capase-3: Cysteiny aspartate specific proteinase-3; mRNA: Messenger RNA; Ang: Angiotensin; SSYX: Shensong Yangxin Capsule; NRCMs: Neonatal rat cardiomyocytes; SD: Standard deviation.

Figure 1: Myocardial hypertrophy and apoptosis in the control, AngII, SSYX (0.25, 0.5, and 1.0 μg/ml drug concentration) groups. (a) Representative immunofluorescent (α-actinin) images of NRCMs in each group. Bar = 200 μm. Red staining denotes α-actinin-stained cardiomyocytes. Blue staining denotes DAPI-stained myocardial cell nuclei. (b) Summarized data of surface area of myocardial cells in each group. (c) Relative expression levels of <i>BNP</i> mRNA in each group. (d) Relative expression levels of <i>Capase-3</i> mRNA in each group. *<i>P</i> < 0.0001 versus the control group; <sup>†</sup><i>P</i> < 0.05 versus the AngII group. The data are expressed as the mean ± SD (<i>n</i> = 5, samples were collected from five different passages). The symbols “*, <sup>†</sup>” indicate that the value of cell volume and <i>BNP</i> and <i>Capase-3</i> mRNA expression significantly differed from different groups. <i>BNP</i>: Brain natriuretic peptide; <i>Capase-3</i>: Cysteiny aspartate specific proteinase-3; mRNA: Messenger RNA; Ang: Angiotensin; SSYX: Shensong Yangxin Capsule; NRCMs: Neonatal rat cardiomyocytes; SD: Standard deviation.