Figure 4: SIR did not result in large generation of ROS after SI treatment. ROS levels of SI and SIR-0.5 h groups in the models 1–6 using H9c2 cells. FITC-positive percentage of cells refers to the FITC-positive cells/total cells; geometric mean refers to the geometric mean of FITC fluorescence intensity. Data are presented as mean ± SD (n = 3–4). *P < 0.05, †P < 0.01 compared to control group; ‡P < 0.05 compared to SI group with the same model. SI: Simulated ischemia; SIR: Simulated ischemia-reperfusion; ROS: Reactive oxygen species; FITC: Fluorescein isothiocyanate; NS: No significance.