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ORIGINAL ARTICLE
Year : 2018  |  Volume : 131  |  Issue : 4  |  Page : 402-412

Moderate Dose of Trolox Preventing the Deleterious Effects of Wi-Fi Radiation on Spermatozoa In vitro through Reduction of Oxidative Stress Damage


1 Department of Urology, School of Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China
2 Department of Andrology, Shaanxi Maternal and Child Care Service Center, Xi'an, Shaanxi 710061, China
3 Research Center of Reproduction Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China
4 Department of Biochemistry, Institute of Biochemistry and Molecular Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China

Correspondence Address:
Dr. Jun-Ping Xing
Department of Urology, School of Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an, Shaanxi 710061
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0366-6999.225045

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Background: The worsening of semen quality, due to the application of Wi-Fi, can be ameliorated by Vitamin E. This study aimed to demonstrate whether a moderate dose of trolox, a new Vitamin E, inhibits oxidative damage on sperms in vitro after exposure to Wi-Fi radiation. Methods: Each of the twenty qualified semen, gathered from June to October 2014 in eugenics clinic, was separated into four aliquots, including sham, Wi-Fi-exposed, Wi-Fi plus 5 mmol/L trolox, and Wi-Fi plus 10 mmol/L trolox groups. At 0 min, all baseline parameters of the 20 samples were measured in sequence. Reactive oxygen species, glutathione, and superoxide dismutase were evaluated in the four aliquots at 45 and 90 min, as were sperm DNA fragments, sperm mitochondrial potential, relative amplification of sperm mitochondrial DNA, sperm vitality, and progressive and immotility sperm. The parameters were analyzed by one-way analysis of variance and Tukey's posttest. Results: Among Wi-Fi plus 5 mmol/L trolox, Wi-Fi-exposed and Wi-Fi plus 10 mmol/L trolox groups, reactive oxygen species levels (45 min: 3.80 ± 0.41 RLU·10−6·ml−1 vs. 7.50 ± 0.35 RLU·10−6·ml−1 vs. 6.70 ± 0.47 RLU·10−6·ml−1, P < 0.001; 90 min: 5.40 ± 0.21 RLU·10−6·ml−1 vs. 10.10 ± 0.31 RLU·10−6·ml−1 vs. 7.00 ± 0.42 RLU·10−6·ml−1, P < 0.001, respectively), percentages of tail DNA (45 min: 16.8 ± 2.0% vs. 31.9 ± 2.5% vs. 61.3 ± 1.6%, P < 0.001; 90 min: 19.7 ± 1.5% vs. 73.7 ± 1.3% vs. 73.1 ± 1.1%, P < 0.001, respectively), 8-hydroxy-2'-deoxyguanosine (45 min: 51.89 ± 1.46 pg/ml vs. 104.89 ± 2.19 pg/ml vs. 106.11 ± 1.81 pg/ml , P = 0.012; 90 min: 79.96 ± 1.73 pg/ml vs. 141.73 ± 2.90 pg/ml vs. 139.06 ± 2.79 pg/ml; P < 0.001), and percentages of immotility sperm (45 min: 27.7 ± 2.7% vs. 41.7 ± 2.2% vs. 41.7 ± 2.5%; 90 min: 29.9 ± 3.3% vs. 58.9 ± 4.0% vs. 63.1 ± 4.0%; all P < 0.001) were lowest, and glutathione peroxidase (45 min: 60.50 ± 1.54 U/ml vs. 37.09 ± 1.77 U/ml vs. 28.18 ± 1.06 U/ml; 90 min: 44.61 ± 1.23 U/ml vs. 16.86 ± 0.93 U/ml vs. 29.94 ± 1.56 U/ml; all P < 0.001), percentages of head DNA (45 min: 83.2 ± 2.0% vs. 68.2 ± 2.5% vs. 38.8 ± 1.6%; 90 min: 80.3 ± 1.5% vs. 26.3 ± 1.3% vs. 26.9 ± 1.1%; all P < 0.001), percentages of sperm vitality (45 min: 89.5 ± 1.6% vs. 70.7 ± 3.1% vs. 57.7 ± 2.4%; 90 min: 80.8 ± 2.2% vs. 40.4 ± 4.0% vs. 34.7 ± 3.9%; all P < 0.001), and progressive sperm (45 min: 69.3 ± 2.7% vs. 55.8 ± 2.2% vs. 55.4 ± 2.5%; 90 min: 67.2 ± 3.3% vs. 38.2 ± 4.0% vs. 33.9 ± 4.0%; all P < 0.001) were highest in Wi-Fi plus 5 mmol/L trolox group at 45 and 90 min, respectively. Other parameters were not affected, while the sham group maintained the baseline. Conclusion: This study found that 5 mmol/L trolox protected the Wi-Fi-exposed semen in vitro from the damage of electromagnetic radiation-induced oxidative stress.

 

 Abstract in Chinese

中等剂量的 trolox 通过减少氧化应激降低 Wi-Fi 电磁辐射对体外精子的损伤

摘要

背景: 维生素E (生育酚) 可以改善Wi-Fi辐射对精液质量的不良影响。 本研究旨在研究中等剂量的 trolox 是否能够通过抑制氧化损伤保护暴露在Wi-Fi电磁辐射的体外精子。

方法: 2014年6月到10月间, 在优生门诊收集20份合格精液标本, 将每一份标本分为假暴露组, Wi-Fi 暴露组, Wi-Fi加5 mmol/L trolox组和Wi-Fi加10 mmol/L trolox组。评估20份精液标本的基线参数。分别在45 和90分钟评估活性氧, 谷胱甘肽过氧化物酶和超氧化物歧化酶的含量, 精子DNA碎片, 精子线粒体膜电位, 精子线粒体DNA相对扩增情况, 以及精子活率, 前向运动精子和不动精子比率。 以上参数通过单因素方差分析和事后Tukey检验进行比较分析。

结果: 在Wi-Fi 加5 mmol/L trolox组、Wi-Fi暴露组和Wi-Fi加10 mmol/L trolox组中,Wi-Fi 加5 mmol/L trolox组在45和90分钟的, 活性氧水平 (45分钟: 3.80 ± 0.41 RLU·10−6·ml−1 vs. 7.50 ± 0.35 RLU·10−6·ml−1 vs. 6.70 ± 0.47 RLU·10−6·ml−1, P < 0.001; 90分钟: 5.40 ± 0.21 RLU·10−6·ml−1 vs. 10.10 ± 0.31 RLU·10−6·ml−1 vs. 7.00 ± 0.42 RLU·10−6·ml−1, P < 0.001), 尾部DNA百分比 (45分钟: 16.8 ± 2.0% vs. 31.9 ± 2.5% vs. 61.3 ± 1.6%, P < 0.001; 90分钟: 19.7 ± 1.5% vs. 73.7 ± 1.3% vs. 73.1 ± 1.1%, P < 0.001), 8羟化脱氧鸟苷含量 (45分钟: 51.89 ± 1.46 pg/ml vs. 104.89 ± 2.19 pg/ml vs. 106.11 ± 1.81 pg/ml, P = 0.012; 90分钟: 79.96 ± 1.73 pg/ml vs. 141.73 ± 2.90 pg/ml vs. 139.06 ± 2.79 pg/ml; P < 0.001) 和不动精子比率 (45分钟: 27.7 ± 2.7% vs. 41.7 ± 2.2% vs. 41.7 ± 2.5%; 90分钟: 29.9 ± 3.3% vs. 58.9 ± 4.0% vs. 63.1 ± 4.0%; all P < 0.001) 均最低; 谷胱甘肽过氧化物酶 (45分钟: 60.50 ± 1.54 U/ml vs. 37.09 ± 1.77 U/ml vs. 28.18 ± 1.06 U/ml; 90分钟: 44.61 ± 1.23 U/ml vs. 16.86 ± 0.93 U/ml vs. 29.94 ± 1.56 U/ml; P < 0.001), 头部DNA百分比 (45分钟: 83.2 ± 2.0% vs. 68.2 ± 2.5% vs. 38.8 ± 1.6%; 90分钟: 80.3 ± 1.5% vs. 26.3 ± 1.3% vs. 26.9 ± 1.1%; P < 0.001), 精子活率 (45分钟: 89.5 ± 1.6% vs. 70.7 ± 3.1% vs. 57.7 ± 2.4%; 90分钟: 80.8 ± 2.2% vs. 40.4 ± 4.0% vs. 34.7 ± 3.9%; P < 0.001) 和前向运动精子比率 (45分钟: 69.3 ± 2.7% vs. 55.8 ± 2.2% vs. 55.4 ± 2.5%; 90分钟: 67.2 ± 3.3% vs. 38.2 ± 4.0% vs. 33.9 ± 4.0%; P < 0.001) 均最高。其他的参数没有明显变化,假暴露组的参数保持基线水平不变。

结论: 5 mmol/L trolox能够保护体外Wi-Fi暴露的精液免受电磁辐射引起的氧化应激损害。



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