Effects of Matricaria chamomilla Extract on Growth and Maturation of Isolated Mouse Ovarian Follicles in a Three-dimensional Culture System
Hamed Shoorei1, Arash Khaki2, Nava Ainehchi3, Mohammad Mehdi Hassanzadeh Taheri4, Moloud Tahmasebi5, Giti Seyedghiasi3, Ziba Ghoreishi6, Majid Shokoohi1, Amir Afshin Khaki3, Sayed Haidar Abbas Raza7
1 Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 Women's Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4 Department of Anatomical Sciences, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
5 Department of Anatomical Sciences, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
6 Department of Nursing, Faculty of Paramedical, Mashhad University of Medical Sciences, Mashhad, Iran
7 Department of Biology, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
Dr. Arash Khaki
Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz
Source of Support: None, Conflict of Interest: None
Background: The aim of this study was to design and assess the effects of hydroalcoholic extract of Matricaria chamomilla (MC) on preantral follicle culture of mouse ovaries in a three-dimensional culture system.
Methods: Isolated preantral follicles were randomly divided into three main groups: the control group containing 10% fetal bovine serum without MC extract (G1), the first experimental group supplemented with 25 μg/ml hydroalcoholic extract of chamomile (G2), and the second experimental group supplemented with 50 μg/ml hydroalcoholic extract of chamomile (G3).
Results: After 12 days of culture, the survival rate (P < 0.05), antrum formation (P < 0.01), metaphase two oocytes (P < 0.01), and the expression of PCNA (P < 0.05) and FSHR (P < 0.05) genes significantly decreased in G3 as compared with G1. On the other hand, at the last day of culture (day 12), the mean diameter of follicles cultured in the medium which was supplemented with 50 μg/ml hydroalcoholic extract of chamomile significantly decreased as compared with the G1 (P < 0.05). In addition, the levels of progesterone and dehydroepiandrosterone hormones significantly increased in the medium of G3 relative to G1 (P < 0.01), while in the medium of G1, the level of 17β-estradiol was significantly higher than that of other groups (P < 0.01). Reactive oxygen species levels of metaphase II oocytes were significantly decreased in G2 as compared with G1 (P < 0.01).
Conclusion: Adding chamomile extract to culture media appeared to decrease follicular function and development.